These are my (incomplete and possibly inaccurate) notes on the enzymes found in Aspergillus Oryzae. Will be updated occasionally.
Enzyme | Optimal Activity Temp (â) | Optimal Activity pH | Mineral required? | Notes | Papers |
---|---|---|---|---|---|
âş-Amylases | 50-70 | 4-5 | 50ppm calcium | endo-1,4-D-glucan glucohydrolase; extracellular; cuts starches at random places; requires atleast 3 glucose units in length; | https://sci-hub.tw//10.1016/S1369-703X(02)00192-4https://s3.wp.wsu.edu/uploads/sites/1254/2016/05/tang83.pdf; Fennema |
Glucoamylase (amyloglucosidase, đ-amylase) | 55-60 | 3.5-4.5 | Â | exo-1,4-âş-D-glucan glucanhydrolase | Fennema |
lipase | 24 | Â | Â | Â | https://www.sciencedirect.com/science/article/abs/pii/0922338X94901163 |
lactase (β-galactosidase) | 55-60 | 2.5-5.0 |  | Most lactase needs Mg, not fungi though. | https://pdfs.semanticscholar.org/f189/0e827656af38c55fa417590cf38da242280e.pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1002/bit.260240207https://sci-hub.tw/10.1016/0168-1656(90)90065-Jhttps://sci-hub.tw/10.1111/j.1365-2621.1979.tb10016.x |
β-glucosidase | 50 | 4.0 |  | cuts terminal glucose in cellulose | https://www.sciencedirect.com/science/article/abs/pii/S1389172302800950; https://sci-hub.tw/10.1016/S1389-1723(02)80095-0 |
endo-1,4-β-glucanases | 50-60 | 3.5-4.5 |  | cuts cellulose in the middle | https://www.sciencedirect.com/science/article/abs/pii/S1389172302800950https://sci-hub.tw/10.1016/S1389-1723(02)80095-0 |
tanninase | Â | Â | Â | Â | Â |
acid protease | 37 (need more research) | 4.0 | Â | Â | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1165373/https://onlinelibrary.wiley.com/doi/full/10.1002/jib.427 |
alkaline protease (Oryzin) | Â | Â | Â | predominant extracellular alkaline endopeptidase; serine | Â |
all proteases | 45-60 | Â | Â | activity drops quickly with more salt, and prolonged time at temperatures above 55C | https://sci-hub.tw/10.1021/jf0486390 |
glutaminase | Â | Â | Â | Â | Â |
keratinase | Â | Â | Â | Â | Â |
limit-dextrinase (pullulanase) | Â | Â | Â | cuts the alpha-1,6 bonds in branched dextrins (from amylopectin) | Â |